What might you observe
Immunoblotting was performed by incubating the blocked membrane overnight at 4 C with the following antibodies: monoclonal anti-cleaved caspase-3 (Cell signaling/#9661, Danver, MA, USA), anti-phospho-protein kinase RNA-like endoplasmic reticulum kinase (p-PERK) (Cell signaling/#3179), activating transcription factor 4 (anti-ATF4)(Cell signaling/#11,815), anti-HSP70 (Cell signaling/#4872), anti-HSP60 (Cell signaling/#4870), anti-ATP-dependent Clp protease proteolytic subunit (ClpP)(Abcam/ab124822, Cambridge,MA, USA), anti-SREBP1 (Thermo/PA1337, Waltham,MA, USA), anti-enoyl-CoA hydratase (ECHS1) (Protein- tech/11305,Rosemont, IL, USA),and anti-GAPDH antibody (Thermo/MA-15738)
The TRPA1 ion channel mediates oxidative stress-related migraine pathogenesis
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